BIOLOGICAL PREPARATION AND METHOD OF REMOVING CRUDE OIL AND PETROLEUM-PRODUCT CONTAMINANTS FROM WATER AND SOIL

摘要

The proposed biological preparation for removing crude oil and petroleum-product contaminants from water and soil consists of a mixture of the following hydrocarbon-oxidising strains of micro-organism: rhodococcus sp. BKM-AC-1500 D, rhodococcus maris BKM-AC-1501 D, rhodococcus erythropolis BKM-AC-1502 D, pseudomonas stutzeri BKM-B-1972 D, Candida sp. BKM-Y-2778 D. The relative proportions of these strains are (0-1):(0-1):(0-1):(0-1):(0-1). The prepar ation in question is a naturally occurring association of microorganisms or mixture of individual micro-organism strains. The proposed method of removing crude oil and petroleum-product contaminants from water and soil involves treating the water or soil with the biological preparation containing the strains rh. sp. BKM-AC-1500 D, rh. maris BKM-AC-1501 D, rh. erythropolis BKM-AC-1502 D, ps. stutzeri BKM-B-1972 D, and C. sp. BKM-Y-2778 D. The preparation used contains no fewer than 2 x 10<13> live microbial cells for every square metre of contaminated area. Treatment of the water and/or soil is carried out by sprinkling a suspension of the preparation in a solution of mineral salts. For the treatment of soil, trenches are cut in the soil. Water and/or soil with a salinity of up to 10 % is treated in this way. The preparation can be used for treating reservoirs and saline soil and brackish water ecosystems.