| 摘要 |
A method for detecting an analyte in a sample uses both the specificity of an enzymatic reaction and the separation power of capillary electrophoresis. In general, the method comprises: (1) subjecting a first aliquot of the sample to an analytical technique such as capillary electrophoresis, which generates a first output such as an electropherogram; (2) reacting a second aliquot of the sample in an enzyme-catalyzed reaction converting the analyte into a product, the product being detectable by the analytical technique; (3) subjecting the second aliquot to the analytical technique to generate a second output; (4) in the case of electrophoresis, measuring the absorbance of the first and second outputs (electropherograms) as a function of migration distance along the electropherogram at at least one wavelength at which either the analyte or the product absorbs to produce a first absorbance scan and a second absorbance scan; and (5) comparing the first absorbance scan with the second absorbance scan to detect the analyte. The reaction can involve a coenzyme and the analytical technique can be directed to the coenzyme. Alternatively, at least two enzymes can be used, the first enzyme generating a first product that is then acted upon by the second enzyme. |
