MULTICLONING VECTOR, EXPRESSION VECTOR, AND PRODUCTION OF FOREIGN PROTEIN WITH EXPRESSION VECTOR.

摘要

An expression vector capable of expressing in a fission yeast Schizosaccharomyces pombe; a multicloning vector for constructing the expression vector; a method of constructing the expression vector; a transformant holding the expression vector; and a process for producing a foreign protein by using the transformant. The multicloning vector has a sequence of -ACATGT-starting with the 5' side at the tip of the multicloning site as the restriction enzyme recognition site for introducing a structural gene of a foreign protein. An expression vector is constructed by introducing a structural gene of a foreign protein by using the above recognition site in such a manner that the tip of the structural gene will have a sequence of -ACATGN- (N being an arbitrary base) starting with the 5' side and that the ATG region of the sequence will function as a translation initiation site of the structural gene. Since it is possible to use an arbitrary base as the N in the sequence of ATGN of the expression vector, it becomes possible to introduce a structural gene of any foreign protein and to express the structural gene to a high level. <IMAGE>