GLYCOPROTEIN DERIVED FROM HUMAN, PHYSIOLOGICALLY ACTIVE FACTOR COMPOSED OF THE SAME GLYCOPROTEIN AND PHARMACEUTICAL PREPARATION CONTAINING THE SAME ACTIVE INGREDIENT

摘要

PURPOSE:To obtain a new glycoprotein, available from a culture supernatant of a fibroblast derived from a human and useful as an antltumor agent, a cell immunopotentiator, etc. CONSTITUTION:This glycoprotein is obtained from a culture supernatant of a fibroblast derived from a human and has the following properties: molecular weight: 78000+ or -2000 or 74000(+)2000 in a nonreduced state and a common band (A) of 52000(+)2000 and two bands of a band (B) of 30000+ or -2000 and a band (C) of 26000+ or -2000 in a reduced state (measured by the SDS-polyacrylamide electrophoresis); isoelectric point: 7.4-8.6; thermal stability:stable even by heating at 60 deg.C for 10min; pH stability: stable within the range of pH6-9; saccharide chain: manifesting the adsorptivity for concanavalin A-Sepharosce(R); physiological activity: capable of suppressing the proliferation of a KB cell, an HeLa cell and an L-929 cell without suppressing the proliferation of an IMR-90 cell; reactivity to antibody: without neutralizing the disorder activity by an anti-TNF antibody, an antilymphotoxin antibody and an antiinterferon beta antibody and amino acid sequence at the N-terminal; formula I or II (X is unidentified). Furthermore, the protein is obtained by a genetic engineering technique.