N4- acylated cytidinyl compounds useful in oligonucleotide synthesis

摘要

Disclosed herein are protecting groups for exocyclic amino groups of the bases adenine, guanine and cytosine for use in the synthesis of oligonucleotides, the protecting groups being represented by the formula: -CO- (CH2)0-9-CH3. In a particularly preferred embodiment, the base cytosine is protected with acetyl (-CO-CH3), and the oligonucleotide incorporating said protected cytosine is subjected to a cleavage/deprotection reagent comprising at least one straight chain alkylamine having from 1 to about 10 carbon atoms.