Potentiometric biosensors, control and applications thereof

摘要

The present invention relates generally to a potentiometric alternating biosensor system (PAB) which can use a silicon based light-addressable transducer to measure parameters such as pH and redox potential. A first aspect relates to temperature control of a PAB system. A second aspect relates to a biosensor for measuring pH and/or redox potential variations in enzymic reactions associated with cells. A third aspect relates to a silicon transducer redox potential biosensor having deposited thereon a metal layer over at least a portion of the surface, and its use in a PAB to monitor enzymes catalyzing redox reactions. A fourth aspect relates to a 2D PAB system capable of simultaneous pH and redox potential measurement. A fifth aspect relates to use of a PAB system for measurement of extracellular acidification to monitor cell metabolism and its use for testing anti-cancer activity/toxicity of compounds on cells. A sixth aspect relates to a PAB system capable of acting as an immunosensor in an immunoassay by virtue of the application to the transducer of an immobilized monolayer of an enzyme.