Monoclonal antibodies against hair follicle, hybridomas producing the antibodies, and a method for preparing the hybridomas

摘要

This invention provides a method for preparing hybridomas, which comprises the steps of: quickly and deeply freezing hair-bulb of follicle isolated from a human or an animal of species A; immunizing an animal of species B by subcutaneously administering thawed hair-bulb to said animal; re-immunizing said animal by intravenously and intraperitoneally administering liquid and solid components of the hair-bulb which are obtained with centrifugation of the thawed hair-bulb to said animal; fusing spleen cells or lymphoid cells isolated from the immunized animal with myeloma cells derived from an animal of species B or C, and cloning fusion cells which produce antibodies capable of specifically binding to the hair follicle of human or animal of species A. The hybridomas prepared by the above method can produce monoclonal antibodies specifically recognizing hair follicle tissues, and these antibodies enable functional analysis of hair follicle in addition to development of new diagnostic and therapeutic means for abnormal hair development, alopecia, etc.