A NEW CEPHALOSPORIN C ACYLASE

摘要

A mutant CC acylase wherein at least one amino acid at the A1a<49>, Met<164>, Ser<166>, Met<174>, Glu<358>, Met<465>, Met<506>, or Met<750> position of the animo acid sequence of the native CC acylase is replaced by a different amino acid, a DNA coding therefor, an expression vector containing the said DNA, a microorganism transformed with the said expression vector, the production of the CC acylase by culturing the said transformant, and use thereof for the production of a compound. The mutant CC acylase of the invention has desirable properties in terms of enzymatic potency, alteration of pH profile, efficiency of processing, and the like.