发明名称 |
Method for refolding insoluble, misfolded insulin-like growth factor-1 into an active conformation |
摘要 |
A method for refolding insoluble, improperly folded IGF-I is provided, wherein the IGF-I, precipitated from prokaryotic host cells, is concurrently solubilized, unfolded, and refolded into a biologically active conformation in a single buffer. The buffer contains reducing agent and chaotropic agent to solubilize the IGF-I at concentrations sufficiently low to allow solubilization and refolding to occur. Also provided is a triple-protease deficient E. coli host suitable for use in the process.
|
申请公布号 |
US5410026(A) |
申请公布日期 |
1995.04.25 |
申请号 |
US19930161044 |
申请日期 |
1993.12.02 |
申请人 |
GENENTECH, INC. |
发明人 |
CHANG, JUDY Y.;MCFARLAND, NANCY C.;SWARTZ, JAMES R. |
分类号 |
C07K1/02;C07K1/113;C07K14/575;C07K14/65;C12N1/21;C12N15/00;C12N15/16;C12P21/02;C12R1/19;(IPC1-7):C12N15/00;C07K7/04 |
主分类号 |
C07K1/02 |
代理机构 |
|
代理人 |
|
主权项 |
|
地址 |
|