摘要 |
PURPOSE:To remove an interfering substance adversely affecting the measurement of sugar-alcohol via simple operation, and improve the measurement accuracy thereof by using a pre-treatment column of specific constitution. CONSTITUTION:An interfering substance removal column used for pre-treatment supplies a bio-originated sample presumed to contain 1,5-anhydroglucitol (1, 5-AG), together with transfer liquid 1. In addition, strong base ion exchange resin to be charged into the column has a grain size of 100mum per wave and at least a part of the grains has ion exchange capacity equal to or above 0.7eq/ml. Also, total ion exchange capacity is equal to or above 0.4meq and removal limiting molecular weight is equal to or less than 50,000. For the measurement of 1, 5-AG as sugar-alcohol, the transfer liquid 1 is carried on the operation of a pump 3, and supplied to an ion exchange resin filled column 6, together with a sample with a sample filling device 5, thereby removing a sugar ingredient and protein. The liquid after the column 6 is made to join a transfer liquid 2, and supplied to a reactor column 7 on the operation of a pump 4, thereby detecting post-reaction absorbance at a process 8 for treatment at a process 9. |