| 摘要 |
The hypervariable region of human DNA due to the tandemly repeated DNA is useful in forensic identity test and human genetics. The alleles of such DNA have been identified by sizing by the gel electrophoresis either after restriction digestion or after amplification by the polymerase chain reaction. We have developed an easy and faithful alternative for identification of alleles of the tandemly repeated DNA based on the allele-specific hybridization and ELISA technology. An aliquot of PCR-amplified digoxygenin-labeled sample DNA is mixed with a set of biotin-labeled standard DNA and the mixture is denatured and then renatured. Only those duplex DNA made of one strand from the sample and the other strand from the standard can be immobilized to the avidin-coated solid support and be attached by the antibody-conjugated enzyme producing visible precipitate. From the position of color development, the alleles in the sample are determined. |
