发明名称 |
METHOD OF SELECTING TRANSFORMATION CELL |
摘要 |
Methods are provided for producing protein products in host cells and for selecting transformed cells comprising the step of transforming the host cell with a DNA molecule comprising a gene which complements a deficiency in the host cell. The host cell is a strain having a deficiency in a function necessary for normal cell growth. The gene in the DNA molecule, such as a plasmid, which complements the deficiency serves as a selection maker whereby the growth conditions for selection may comprise a conventional complex medium. |
申请公布号 |
JPH0767685(A) |
申请公布日期 |
1995.03.14 |
申请号 |
JP19940118650 |
申请日期 |
1994.05.31 |
申请人 |
ZYMOGENETICS INC |
发明人 |
GUREN KAWASAKI;RESURII BERU |
分类号 |
C12N15/09;C07H21/04;C07K14/15;C07K14/395;C07K14/49;C07K14/81;C12N1/16;C12N1/19;C12N5/00;C12N9/90;C12N15/00;C12N15/64;C12N15/67;C12N15/81;C12P19/34;C12P21/00;C12P21/02;C12R1/645;C12R1/865;(IPC1-7):C12P21/02 |
主分类号 |
C12N15/09 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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