发明名称 |
FACTOR FOR FIBROBLAST COLONY FORMING UNIT |
摘要 |
A colony forming unit fibroblast (CFU-F) factor has been identified having the following characteristics: (a) it is a polypeptide present in P388 or 416B (ECACC provisional accession no. 94082401) cells and capable of extraction from the cell lysate or conditioned medium; (b) when added to explanted bone marrow adherent cells which are below the level capable of forming fibroblast colonies, it is capable of inducing the formation of fibroblast colonies; (c) it binds heparin-agarose at physiological pH; (d) the heparin binding fraction, when subjected to reducing SDS-PAGE, produces bands at about (12, 15, 16 and 17kD); (e) its activity is not affected by heating at 100 .degree.C for 10 minutes; (f) it can be purified from the cell lysate by fractionation by anion-exchange chromatography, eluting at 0.3M sodium chloride; followed by size exclusion chromatography, optionally followed by further purification such as HPLC and/or SDS-PAGE.
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申请公布号 |
CA2169945(A1) |
申请公布日期 |
1995.03.02 |
申请号 |
CA19942169945 |
申请日期 |
1994.08.26 |
申请人 |
CANCER RESEARCH CAMPAIGN TECHNOLOGY LIMITED |
发明人 |
JONES, SARAH JANE;MOLINEUX, GRAHAM;DEXTER, MICHAEL THOMAS;FRIEDENSTEIN, ALEXANDER |
分类号 |
C12P21/00;A61K35/14;A61K38/00;A61P43/00;C07K1/16;C07K1/18;C07K1/22;C07K14/475;C07K14/50;C12R1/91;(IPC1-7):C12P21/00 |
主分类号 |
C12P21/00 |
代理机构 |
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地址 |
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