Diagnostic compositions, elements, methods & test kits for amplification & detection of two or more DNA's using primers having matched melting temperatures.

摘要

An aqueous composition containing primers for opposing strands of two or more target nucleic acids can be used in polymerase chain reaction to provide simultaneously rapid and efficient amplification and detection of those nucleic acids. The primers for each target DNA differ in length by no more than 5 nucleotides and have a Tm within the range of from 65 to 74 DEG C, while the Tm's are within about 5 DEG C of each other. Such compositions are useful in diagnostic test kits and methods for amplification and detection of multiple nucleic acids, or in "multiplexing", using multiple capture probes. All of the capture probes have Tm's which are greater than 50 DEG C and are within 15 DEG C of each other.