| 摘要 |
<p>A method for determining the extent of an infection in a human or animal patient by detecting the amount of an antigen indicative of such infection, the amount of the antigen is detected in a patient blood derived test sample containing blood cell fractions, the method comprises: i) incubating the test sample with an amount of test antibodies specific to the antigen indicative of infection to form antibody/antigen complexes which are insoluble in the sample, ii) allowing the antibody/antigen complexes to interact with the white blood cell fractions which results in the production of oxidants, iii) introducing to either steps i) or ii) a chemiluminescent compound to the test sample, iv) the oxidants reacting with the chemiluminescent compounds to emit luminescent light from the test sample, v) measuring the amount of emitted light over a predetermined period, and vi) correlating extent of infection by comparison of the measured amount of emitted light of the test sample with measured amount of light emitted by a control sample which is treated the same as the test sample for steps i) to v) except that in step i) control antibodies are used which are of the same class as the test antibodies but are non-specific to the antigens indicative of infection.</p> |
