| 摘要 |
The method according to the invention consists in a) adding to the medium to be analyzed a compound of formula (I), A and C, which are the same or different, are each H, ( alpha ) or ( beta ) but not together H; and B= ( psi ), ( delta ), ( epsilon ), ( phi ), ( gamma ), ( eta ), ( iota , ( xi ) or ( kappa ); b) acidifying the medium, either with the aid of a strong carboxylic or sulfonic acid or perchloric acid, or with hydrochloric, hydrobromic or sulfuric acid; c) incubating the acidified medium between 25 and 60 DEG C to obtain a stable coloration; d) measuring the absorbency of the coloured medium at the absorption wavelength which is specific to the chromophoric additive formed and using the value of this measurement to determine the total concentration of 4-hydroxy-2-enaldehydes and MDA, or malonic dialdehyde only, according to the acid used. Application in the analysis of the indexes of lipid peroxidation, especially in clinical research. |
