| 摘要 |
<p>A method for generating monoclonal antibodies directed against previously uncharacterized an unpurified antigens on the surface of target cells in a cell population. The method includes incubating (100) a combinatorial library of antibodies expressed on the surface of filamentous phage particles with a target cell population under conditions sufficient to bind a portion of the phage particles to the target cells. The target cells and bound phage particles are then separated (102) from the unbound phage particles, and the bound phage particles are recovered (104). These phage particles are then amplified (106) to create an enriched library. Monoclonal antibodies specific to the target cell are then isolated (108) from the enriched library for subsequent use.</p> |
