Oligonucleotides with selectably cleavable and/or abasic sites

摘要

A modified polynucleotide containing at least one cleavable or abasic site as shown below. <IMAGE> DNA1 is a first segment of DNA; DNA2 is a second segment of DNA; and Rm is C1 to C16 alkylene or an oxytheylene oligomer -(CH2CH2O)z- where z is an interger in the range of 1 to 16 inclusive, and Rn is selected from the group consisting of <IMAGE> <IMAGE> <IMAGE> <IMAGE> <IMAGE> CH3OCH2CH2OCH2. Such polynucleotides are useful in solid phase hybridizations because they permit the release of a label from the solid support after the hybridization reaction.