发明名称 |
NEW GLUCOSE ISOMERASE HAVING MODIFIED AND OPTIMIZED PH |
摘要 |
A method for selecting amino acid residues is disclosed which upon replacement will give rise to an enzyme with an altered pH optimum. The method is specific for metalloenzymes which are inactivated at low pH due to the dissociation of the metal ions. The method is based on altering the pKa of the metal coordinating ligands or altering the Kass for the metal binding. New glucose isomerases with an altered pH optimum are provided according to this method. These altered properties enable starch degradation to be performed at lower pH values. |
申请公布号 |
JPH06319543(A) |
申请公布日期 |
1994.11.22 |
申请号 |
JP19910187106 |
申请日期 |
1991.01.04 |
申请人 |
GIST BROCADES NV;PLANT JIENETEITSUKU SYST NV |
发明人 |
AN MARII BUIRUJINIIE RUNE RANBEERU;INIYAASU RASUTERU;NADEIIRU MURABE;UIRUHERUMUSU YOHANESU KUWATSUKUSU;YAN METSUKU FUAN DERU RAAN;ONNO MISETSUTO |
分类号 |
C12N9/00;C12N9/90;C12N9/92;C12N15/09;C12N15/54;C12N15/61;C12P19/02;C12R1/045;C12R1/19 |
主分类号 |
C12N9/00 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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