| 发明名称 |
A METHOD OF PREPARING AN ACTIVE HUMAN NEUTROPHIL CHEMOTACTIC FACTOR POLYPEPTIDE. |
| 摘要 |
<p>A simple and efficient method of preparing active recombinant human neutrophil chemotactic factor. The human neutrophil chemotactic factor (NCF) is solubilized from a transformant cell homogenate using urea or guanidine hydrochloride. The polypeptide is further purified from the solution using conventional protocols such as CM-Sepharose CL-6B, HW-55 column, etc.. By using this method, large amounts of active human NCF can be recovered and purified.</p> |
| 申请公布号 |
EP0431042(B1) |
申请公布日期 |
1994.10.26 |
| 申请号 |
EP19890909925 |
申请日期 |
1989.08.29 |
| 申请人 |
UNITED STATES GOVERNMENT, AS REPRESENTED BY THE DEPARTMENT OF HEALTH & HUMAN SERVICES;DAINIPPON PHARMACEUTICAL CO., LTD. |
发明人 |
YAMADA, MASAAKI 13, MURASAKINO NISHIRENDAINO-CHO;FURUTA, RYUJI;YAMAGISHI, JUNICHI;MATSUSHIMA, KOUJI |
| 分类号 |
C12N15/09;C07K1/14;C07K1/22;C07K14/00;C07K14/52;C07K14/54;C12N15/12;C12P21/00;C12P21/02;C12R1/19;(IPC1-7):C12N15/00 |
主分类号 |
C12N15/09 |
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