发明名称 |
ANTIBODIES AND ASSAYS FOR DETERMINING MUTATIONS IN THE APC GENE |
摘要 |
Antibodies and assays employing them are taught which are useful for detecting the bulk of mutations which occur in the APC gene in familial adenomatous polyposis. The antibodies are specific for epitopes in the amino terminal or carboxy terminal portion of the protein. A variety of immunoassay formats are described which are all based on the observation that the bulk of the APC mutations which occur in familial adebinatous polyposis and sporadic colorectal carcinomas result in truncated APC proteins. Generally, either the size of APC proteins is determined or the relative binding of amino terminal-binding antibodies to carboxy terminal-binding is determined.
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申请公布号 |
CA2158429(A1) |
申请公布日期 |
1994.09.29 |
申请号 |
CA19942158429 |
申请日期 |
1994.03.21 |
申请人 |
JOHNS HOPKINS UNIVERSITY (THE) |
发明人 |
HILL, DAVID E.;JOHNSON, KAREN A.;KINZLER, KENNETH W.;VOGELSTEIN, BERT |
分类号 |
G01N33/574;C07K14/47;C07K16/18;C07K16/22;C07K16/30;C12N5/10;C12N5/20;C12N15/02;C12P21/08;C12R1/91;G01N33/577;(IPC1-7):C12N5/18;G01N33/566 |
主分类号 |
G01N33/574 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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