Detergent comprising isolated cellulase from bacillus ferm bp-3431 or a mutant strain thereof, surfactant and builder

摘要

A cellulase having the following properties: (1) an optimum pH of from 9.5 to 10.5 as measured using carboxymethylcellulose as a substrate; (2) a stable pH of from 6 to 11 as measured using carboxymethylcellulose as a substrate after treating at 30 DEG C. and for 30 minutes; (3) an optimum temperature of about 55 DEG C. as measured using carboxymethylcellulose as a substrate; (4) influence of a surfactant being such that residual activity is 90% or more after treatment at 30 DEG C. and pH of 9.0 for 2 hours in the presence of a sodium n-alkylbenzenesulfonate; (5) a molecular weight of 52,000+/-2,000 as measured by electrophoresis on SDS-polyacrylamide gel; and (6) an isoelectric point of 4.2+/-0.2 as measured by isoelectric electrophoresis. The cellulase is produced by a Bacillus strain FERM BP-3431 or a mutant strain thereof. A detergent comprising the cellulase, a builder and a surfactant is also disclosed.