| 摘要 |
PCT No. PCT/US93/07685 Sec. 371 Date Feb. 14, 1995 Sec. 102(e) Date Feb. 14, 1995 PCT Filed Aug. 16, 1993 PCT Pub. No. WO94/04695 PCT Pub. Date Mar. 3, 1994A replication defective DNA viral vector that comprises a gene of interest encoding a protein having a demonstrated therapeutic effect on a host cell is disclosed. The vector comprises the gene in operable linkage with a neural tissue-specific promoter, which is a promoter derived from a gene normally produced in the host cell. In particular an HSV-1 vector is used. A preferred DNA defective vector is the dvHBENK which includes a promoter prepared from the rat preproenkephalin gene, the promoter being in operable linkage with the lacZ gene. The disclosed DNA viral vector is useful for achieving stable in vivo long-term expression, in particular in mammalian brain cells. |
