| 摘要 |
PURPOSE:To readily obtain a peptide antibody in a large amount at a low cost by preparing the peptide antibody from eggs produced by a chicken preingesting an antigen and providing the peptide antibody rapidly responsive to an undenatured peptide and affording the maximum antibody value in a short time. CONSTITUTION:Leucine enkephalin which is one kind of neuropeptide as an immunogen for a chicken is synthesized by a solid-phase synthetic method, then mixed with a polvinylpyrrolidone-phosphoric acid-buffered physiological saline solution and a complete Freund's adjuvant and emulsified to provide an antigenic liquid, which is subsequently subcutaneously injected into the thigh of the chicken to immunize the chicken. After immunization for the first time, immunizing operation is carried out three times at an interval of 2 weeks. The egg yolk of collected eggs is separated for 9 weeks after the initial immunization and the phosphoric acid buffered-physiological saline solution in an amount equal to that of the egg yolk is added thereto. A 0.1% sodium alginate solution is further added and centrifugation is performed to measure the specific antibody value of the resultant supernatant. An antibody is then collected from a water-soluble fraction in which the antibody value is raised in about the 7th week. Thereby, a large amount of the objective peptide antibody is readily obtained from the eggs produced by the chicken preingesting the antigen at a low cost. |
