| 摘要 |
<p>In a diagnostic procedure for the detection or quantitative determination of antibodies in biological fluids by means of an immunological reaction, in which the sought antibodies are incubated with the corresponding antigen, one portion of which antigen is bound to a water-insoluble carrier, and the other portion of which is provided with a label, after the separation of the phases measuring the extent of the label either in the liquid or in the solid phase, it is possible for components of the analytical sample to bind a labelled antigen to a water-insoluble carrier in an unspecific manner, thus producing incorrect positive results. In order to obtain valid results, the same reaction is conducted but incubation takes place in the presence of an excess of non-bound and non-labelled antigen and, after separation of the phases, the measured extent of the marking is also taken into consideration in the qualitative evaluation or the quantitative analysis of the antibodies. The procedure is particularly suitable for the analysis of human antibodies to rabbit globulin, the antigen being a rabbit globulin.</p> |
