Non-fibrogenic, alginate-coated transplants, process of manufacture and method of use thereof

摘要

A transplant with a core of a viable, physiologically active, cell(s) and a non-fibrogenic coating of alkaline earth metal alginate having a high mannuronate to guluronate molar ratio and free from fibrogenic amounts of fucose, sulfate, phloroglucinol and protein moieties. The coating has a permeability sufficiently low and a thickness sufficiently large to protect the tissue cells from host immunological agents after transplantation, the coating also being sufficiently permeable and thin to permit the diffusion of cell sufficient nutrients and cell products through the coating required for cell viability. The alginate coating can be reacted with polylysine to form a polylysine-alginate complex on the outer surface thereof. The complex can then be reacted with polyaspartic acid to provide a physiologically acceptable negative surface charge.