| 摘要 |
<p>PURPOSE:To obtain a human cellular fibronectin useful as a raw material for additives for a medium of animal cell culture, diagnosticums, cosmetics, and medicines, having activity as cell adhesion factor by culturing variant cell strain HUH-6YM derived from human congenital hepatoma and collecting the product from the culture mixture. CONSTITUTION:Cloning from cell strain HUH-6 clones 5 derived from human congenital hepatoma is repeated, the cell strain is multiplied in a basic medium comprising an amino acid, vitamins, saccharides and inorganic salts, Variant cell strain HUH-6YM derived from human congenital hepatoma which can be limitlessly subjected to subculture without requiring a protein factor as a growth factor and manifests mRNA of fibronectin containing ED-A and ED-B ranges is subjected to subculture at 37 deg.C in air containing 5% CO3 for one month, the cells are inoculated to a medium in a roller bottle, continuously cultured at 37 deg.C under atmospheric pressure for three months, ultrafilterd, concentrated, passed through a gelatin agarose column and purified to give a human cellular fibronectin useful as an additive for a medium in animal cell culture.</p> |
