PREPARATION OF MULTIVALENT VACCINES

摘要

1,270,918. Multivalent vaccines. MERCK & CO., Inc. 9 Nov., 1970 [14 Nov., 1969], No. 53255/70. Heading A5B. A multivalent vaccine may be produced by simultaneously cultivating a number of viral agents in a single cell culture medium and incorporating the culture in a pharmaceutically acceptable carrier. The viral agents may be respitory syncytial virus, parainfluenza viruses, influenza virus strains A and B, infection bronchitis-like viruses and mycoplasma pneumoniae. The culture cells may be African Green Monkey Kidney cells. In Example I the cultured viruses are harvested, inactivated with aqueous formaldehyde and adsorbed on potassium aluminium sulphate. In Example 3 the culture contains neomycin. The virus product is clarified in a centrifuge, fractionated in an ultra-centrifuge and used in aqueous medium.