发明名称 |
Reaction-based selection for expression of and concentration of catalytic moieties |
摘要 |
A recombinant virus or an organism population capable of expression of a surface-bound catalytic moiety is selected by:- reaction-based selection for catalytic activity by a surface-bound catalytic moiety of a population of recombinant viruses or organisms expressing the catalytic moiety, and isolating from said population a subpopulation which can act catalytically, wherein the reaction-based selection comprises contacting said population with a surface including an immobilized hapten or substrate and eluting from said surface said subpopulation which can act catalytically while retaining a population that has binding affinity for the hapten or substrate but no catalytic activity. The method can be used to increase the proportion of catalytic to non-catalytic moieties in a sample. Also provided is a method for producing a recombinant virus or cell line, capable of expression of a surface-bound catalytic moiety and a substantially pure recombinant virus population or cell line. <IMAGE> |
申请公布号 |
AU3776193(A) |
申请公布日期 |
1993.09.13 |
申请号 |
AU19930037761 |
申请日期 |
1993.02.24 |
申请人 |
IGEN, INC. |
发明人 |
MARK T MARTIN;RODGER G SMITH;MICHAEL J DARSLEY;DAVID M SIMPSON;GARY F. BLACKBURN |
分类号 |
G01N33/53;B01J20/281;C12N5/10;C12N7/02;C12N15/10;C12Q1/02;C12Q1/68;C12Q1/70;C40B40/02;G01N30/88;G01N33/569 |
主分类号 |
G01N33/53 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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