摘要 |
In the process for purifying factor VIII from cryoprecipitate, which is dissolved, and which is then treated with alumina gel in the usual manner, - the extract is diluted to a protein concentration less than or equal to about 5 g/l, especially 4 g/l, and subjected to a viral inactivation using solvent/detergent, - then the inactivated extract, containing the solvent/detergent, is subjected to chromatography on a weak anion-exchange column of hydrophilic nature, - the factor VIII then being eluted with a dissociating buffer. The preparation obtained has an activity of the order of at least 100 IU VIII :C/mg of protein. |