摘要 |
<p>This invention relates to a method for isolating immunoglobulins, lactoferrin and lactoperoxidase from milk, in which skimmed, non-sterilized milk is subjected to metal-chelate-interaction-chromatography, comprising the consecutive steps of: a) contacting the milk with a transition metal which is chelated to a functional matrix having a high mechanical strength and a high porosity, as a result of which milk proteins are bonded to the matrix, b) removing the fraction containing milk components not bonded by the matrix, and c) desorbing the proteins bonded to the matrix. The fraction obtained in step b) can be processed to a suitable product by contacting it at least once with a functional matrix as used in step a), only this time not charged with transition metal.</p> |