Method to obtain intact, viable protoplasts from pollen grains

摘要

Intact, viable protoplast are obtained readily and in good quantity from within the cell walls of mature pollen grains. Pollen grains are contacted with an aqueous medium containing an osmolyte, gelatin, or a combination thereof in a concentration to expand the protoplast and force it out through the pore in the cell wall of the pollen grain, while maintaining the protoplast intact and viable.