发明名称 |
ELEMENT AND METHOD FOR NUCLEIC ACID AMPLIFICATION AND DETECTION USING ADHERED PROBES |
摘要 |
An element has been prepared which is useful for the detection of nucleic acids in various formats. The element has a sealable support on which is disposed a nucleic acid reagent composition. The composition is a mixture of a nucleic acid reagent composed of polymeric particles to which an oligonucleotide is covalently attached. The particles are prepared from a first polymer having a glass transition temperature of at least 70 DEG C and have an average diameter of from 0.1 to 3 micrometers. The reagent is adhered to the support using a water insoluble adhesive comprising a second polymer which has a glass transition temperature which is at least 30 DEG C less than the glass transition temperature of the first plymer. The adhesive is present in the composition at from 1 to 20 weight percent. This element provides high sensitivity and low background in hybridization and other nucleic acid assays. |
申请公布号 |
AU1664092(A) |
申请公布日期 |
1992.10.21 |
申请号 |
AU19920016640 |
申请日期 |
1992.03.19 |
申请人 |
EASTMAN KODAK COMPANY |
发明人 |
LEONARD JOSEPH SEABERG;PAUL NICHOLAS SCHNIPELSKY;CHARLES CULLIS HINCKLEY;JEFFREY ALLEN WELLMAN;WILLIAM HAROLD DONISH;JOHN BRUCE FINDLAY;RICHARD CALVIN SUTTON;IGNAZIO SALVATORE PONTICELLO;THOMAS JOSEPH CUMMINS;DENNIS ROLAND ZANDER |
分类号 |
C12N15/09;B01L3/00;C12Q1/68;G01N31/22;G01N33/50;G01N33/52;G01N33/543;G01N33/566;G01N35/00 |
主分类号 |
C12N15/09 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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