| 摘要 |
<p>An improved, highly stable capillary column used in high performance capillary electrophoresis and method of preparing the same. The improved capillary column can significantly reduce the effects of electroosmosis during electrophoresis, is stable for reuse, and enables operation of the capillary column in high electric fields, resulting in high resolution separations in a minimum amount of time. The improved column comprises a capillary (10) having a highly stable, coating matrix (12) on the inner surface (14) of the capillary wall. The highly stable coating matrix includes an intermediate layer hydrophobic phase chemically bonded to the inner wall of the capillary, a detergent which is physically adsorbed to the hydrophobic phase, and a polymerized monomer which is covalently bonded to the adsorbed detergent thereby forming a hydrophilic outer layer of the coating matrix. <IMAGE></p> |
