发明名称 |
SYNTHESIS OF OLIGONUCLEOTIDES |
摘要 |
A method for the synthesis of a plurality of oligonucleotides in which an oligonucleotide is formed by sequential reactions of precursors of individual nucleotides on a support, comprising the steps of (a) forming a first oligonucleotide; (b) attaching to said first oligonucleotide a cleavable linker moiety; (c) forming a second oligonucleotide on the cleavable linker moiety; and (d) optionally cleaving the linker moiety to give the desired oligonucleotides. The invention also concerns nucleoside and non-nucleoside reagents suitable for incorporating cleavable linker moieties during automated oligonucleotide synthesis, cleavage of which produces oligonucleotides having a hydroxy or phosphate group at the 3' and 5' positions, and solid supports suitable for use in automated oligonucleotide synthesisers. |
申请公布号 |
WO9206103(A1) |
申请公布日期 |
1992.04.16 |
申请号 |
WO1991GB01687 |
申请日期 |
1991.10.01 |
申请人 |
IMPERIAL CHEMICAL INDUSTRIES PLC |
发明人 |
HOLLAND, DAVID;GARMAN, ANDREW, JOHN;EDGE, MICHAEL, DEREK;MCLEAN, MICHAEL, JOSEPH |
分类号 |
C07F9/24;C07H21/00;C07H21/04;C12Q1/68 |
主分类号 |
C07F9/24 |
代理机构 |
|
代理人 |
|
主权项 |
|
地址 |
|