发明名称 |
A METHOD FOR OVER-EXPRESSION AND RAPID PURIFICATION OF BIOSYNTHETIC PROTEINS |
摘要 |
The subject invention relates to a method of producing and purifying large quantities of a biosynthetic protein. The gene which codes for the protease is placed between the binding domain of a gene which codes for a binding protein and a gene coding for the target protein of interest. The fused gene construct is inserted in an expression vector which is then introduced into a host cell. |
申请公布号 |
WO9205276(A1) |
申请公布日期 |
1992.04.02 |
申请号 |
WO1991US06735 |
申请日期 |
1991.09.20 |
申请人 |
THE UNITED STATES OF AMERICA, AS REPRESENTED BY TH |
发明人 |
LOUIS, JOHN, M. |
分类号 |
C07K14/16;C07K14/245;C12N9/50;C12N15/62 |
主分类号 |
C07K14/16 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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