| 摘要 |
Inventors: MICHAEL A. SHEARER PAMELA K. SASAGAWA and RONALD H. HEIN Invention: IMPROVED METHOD OF PREPARING ALPHA-1-PROTEINASE INHIBITOR There is disclosed an improved method for separating one of alpha-1-proteinase inhibitor (also known as alpha-1 antitrypsin) and antithrombin-III from an aqueous solution of plasma proteins containing the same, such as from Cohn Fraction IV-1, Cohn Fraction IV, reworks of Cohn Fraction IV and IV-l, Cohn Effluent II /A III and Cohn Effluent I. The method includes the steps of first holding an aqueous solution of plasma proteins containing one of alpha-l-proteinase inhibitor and antithrombin-III in a relatively large volume of buffer solution as solvent and at a pH adjusted to be relatively basic when compared to conditions heretofore known, and at a temperature in the range of from 2 - 50.degree. C for a period of about 0.2 - 24 hours. Following the above treatment, alpha-1-proteinase inhibitor and antithrombin-III are obtained by applying conventional techniques to the resulting solution. Accordingly, the solution is then mixed with a polyalkylene glycol, e.g. polyethylene glycol, in the range of from as low as 8% to as high as 23% (w/v) based on volume of solution to CL-124 selectively precipitate unwanted proteins. The alpha-1-proteinase inhibitor is then separated from the supernatant solution by adsorption on an anion exchange resin or by precipitation by further addition of a polyalkylene glycol, e.g. polyethylene glycol. The method of the invention provides an improvement in the yield of alpha-1-proteinase inhibitor. CL-124 |
