发明名称 |
METHODS OF IDENTIFYING COMPOUNDS THAT ACT AS AGONISTS OR ANTAGONISTS FOR PROTEINS INVOLVED IN SIGNAL TRANSDUCTION |
摘要 |
A method for identifying a chemical that acts as an agonist for a G-protein coupled cell surface (GPC) receptor. The method comprises introducing to test cells of a pigment cell line capable of dispersing or aggregating their pigment in response to a specific stimulus and expressing an exogenous clone coding for the GPC receptor, a stimulant that sets an initial state of pigment disposition wherein the pigment is aggregated within the test cells if activation of the exogenous GPC receptor induces pigment dispersion, or introducing a stimulant that sets an initial state of pigment disposition wherein the pigment is dispersed within the test cells if activation of the exogenous GPC receptor induces pigment aggregation; contacting the test cells set in an initial state of pigment disposition with the test chemical; and determining whether the pigment disposition in the test cells treated with the chemical is changed from the initial state of pigment disposition, wherein a change in pigment disposition observed in the test cells expressing the exogenous GPC receptor indicates that the chemical is an agonist for the exogenous GPC receptor. |
申请公布号 |
WO9201810(A1) |
申请公布日期 |
1992.02.06 |
申请号 |
WO1991US05046 |
申请日期 |
1991.07.17 |
申请人 |
LERNER, MICHAEL, R.;LERNER, ETHAN, A. |
发明人 |
LERNER, MICHAEL, R.;LERNER, ETHAN, A. |
分类号 |
C12Q1/02;C07K14/705;C12N5/10;C12N15/09;C12N15/12;C12N15/85;C12Q1/44;C12Q1/48;C12R1/91;G01N33/50;G01N33/566 |
主分类号 |
C12Q1/02 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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