摘要 |
A process and test device for the determination of an analyte in a body fluid, in which there are used two binding components capable of specifically binding with one another, one of the binding components being enzyme-labelled and not carrier-fixed and the other binding component being carrier-fixed; the binding components are incubated with one another so that a binding reaction takes place; the amount of enzymelabelled binding component not bound to the carrier-fixed binding component being a measure for the concentration of the analyte which is determined by allowing the labelling enzyme to act upon a substrate producing a detection signal; during the specific binding reaction, incubation is carried out simultaneously with a non-fixed substrate of the labelling enzyme which does not produce a detection signal and with a carrier-fixed substrate of the labelling enzyme which produces a detection signal, the substrate not producing a detection signal being so chosen with regard to the amount used and affinity to the labelling enzyme in relation to the amount of the substrate producing a detection signal and its affinity to the labelling enzyme and in relation to the total activity of the labelling enzyme that the enzymecatalyzed reaction of the substrate producing a detection signal is delayed until the specific binding reaction between the binding components has substantially taken place. |