A COMPUTERIZED METHOD OF MATCHING TWO-DIMENSIONAL (2-D) PATTERNS

摘要

A computerized method (200) for use in data acquisition and manipulation of two-dimensional patterns in the fields of medicine, astronomy, chemistry, biology and biotechnology. The interactive computerized method (200) facilitates matching visual patterns of polypeptide spots in two-dimensional gel electrophoretograms solubilized into polypeptide constituents that are separated by electrophoresis. The computerized method (200) manipulates spot pixel coordinates using staged coordinate transformation techniques (251) on spot markers and unknown study spots to reduce gel preparation distortions and allows a user to produce matching results in an operation (252) that compares the tranformed spot data using either a single reference gel approach (255) or a multiple reference gels approach (254) for producing the matching results. The method also includes a spot matching verification step (252a) and a step (256) to extract potentially mismatched spots from reported matching results. The user can also resolve contradictions in resolve step (253) and perform spot matching analysis (270) using isoelectric focusing (PI), and molecular weight (MW) dimensional separation data.