| 摘要 |
The anti-viral transactivating activity of a test substance is determined by (a) putting both the expression of the Secreted Alkaline Phosphatase gene and the viral transactivator gene under the control of a viral promoter responsive to the action of the transactivator, (b) transfecting cultured mammalian cells with plasmids which contain the gene constructs of (a) and cause cellular production of the transactivating factor and Secreted Alkaline Phosphatase (c) adding the substance to be tested, and (d) determining the amount of Secreted Alkaline Phosphatase produced, whereby inhibition of Secreted Alkaline Phosphatase production correlates with anti-viral transactivating activity.
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