摘要 |
The present invention provides a serum-free culture medium for mammalian cells, especially non-adherent cells, containing a base medium, transferrin, insulin and selenite, wherein it contains or consists of 2 to 10 mg./liter transferrin, 2 to 10 mg./liter insulin, 1 to 10 g./liter peptone, 10 to 500 mg./liter beta-D-xylopyranose substituted with phosphate, carboxyl and/or sulphate groups, 0.1 to 0.5 mg./liter selenite and 0.1 to 2 mg./liter biological polyamine.
|