发明名称 |
ACTIVATOR, ACTIVATOR GENE AND MARKER GENE AND PRODUCTION OF USEFUL AND PHYSIOLOGICALLY ACTIVE SUBSTANCE |
摘要 |
NEW MATERIAL:An activator having properties activating a gene derived from a thermophilic bacterium and containing a marker gene and enhancing transfer of structural gene controlled by a gene containing the marker gene. USE:The activator is used for controlling expression of gene concerning production of useful substance or for efficiently producing an useful and physiologically active substance. PREPARATION:For example, Bacillus stearothemophilus is cultured in a culture medium and bacterium cell thereof is collected and chromosome DNA is separated according to an ordinary method and treated with restriction enzymes and then integrated into a vector plasmid separated from Bacillus subtilis MT-2 according to an ordinary method and inserted into Bacillus subtilis MT-2 to transform Bacillus subtilis MT-2 and the transformer is cloned and clone having activating factor gene is selected and cultured to provide the activator. |
申请公布号 |
JPH03108495(A) |
申请公布日期 |
1991.05.08 |
申请号 |
JP19890245894 |
申请日期 |
1989.09.20 |
申请人 |
TOYOBO CO LTD |
发明人 |
NISHIYA YOSHIAKI;SOGABE YUKIHIRO |
分类号 |
C12N15/09;C07K14/00;C07K14/195;C07K14/32;C07K14/41;C12N1/21;C12N15/32;C12P21/02;C12R1/125 |
主分类号 |
C12N15/09 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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