| 摘要 |
NEW MATERIAL:A nucleic acid fragment to code non-A; non-B type hepatitis virus antigen peptide. EXAMPLE:A nucleic acid fragment to code an amino acid sequence shown by the formula. USE:For detecting non-A, non-B type hepatitis virus. PREPARATION:Plasma having high GPT value is concentrated about 1,000 times, whole nucleic acids are purified, DNA derived from human is decomposed, RNA is purified, cDNA is synthesized, inserted into lambdagt11 vector to prepare cDNA library. Escherichia coli is infected with lambda phage, cultured and replica is subjected to immunoscreening to give a clone. |
