| 摘要 |
PURPOSE:To intend breeding improvement industrially by incorporation of gene having a function to stably holding plasmid grouwable through replication in Corynebacteria classified as Brevibacterium sp. CONSTITUTION:Plasmid pBY503 stemmed from Brevibacterium stations 1FO12144 containing fragments of stabilizing DNA (having a function to stably hold plasmid in bacteria) is fragmented using a desired restriction enzyme (e.g. KpnI) to produce DNA fragments (A). The fragments A are then incorporated, in Corynebacteria, into vector plasmid holding instable, medicine-tolerant marker followed by introduction into the Corynebacteria to produce a transformant strain (B). Thence, the plasmid DNA in the B strain is extracted and the objective DNA fragments are collected, which have the following characteristics: size: ca.7.4 Kb; number of recognition site when cut with restriction enzyme EcoRI: 3, and the resulting fragment size being 2.7, 2.5, 2.1, 0.1Kb. |
