A GENE AND GENE STRUCTURE CODING FOR AN AMINOTRANSFERASE,AND MICROORGANISMS WHICH EXPRESS THIS GENE

摘要

The preparation of L-2-amino-4-methylphosphinobutyric acid (L-PPT) by transamination of (3-carboxy-3-oxopropyl)methylphosphinic acid with the aid of L-PPT-specific transaminase from E. coli DH1 becomes very much more efficient when the gene coding for this enzyme is isolated and incorporated in a plasmid, and the latter is used to transform a microorganism. For this purpose, a suitable gene probe was constructed and used to locate the gene on a 3.8 kb BamHI/SalI fragment.