Process for the separation and purification of proteases and antiproteases of blood clotting, as well as of the protease/antiprotease complex

摘要

The present invention relates to a process for separating and purifying proteases and antiproteases. This process is characterized in that there are placed in contact an insoluble cross-linked polymer including in its chain the group -SO3R1- and/or the group -SO2R2- in which R1 denotes a hydrogen atom or metal and R2 denotes the remainder of an amino acid linked to the -SO2- bridge through its amine -NH-, function, with the solution containing proteases and antiproteases or their complex; separating the polymer which has absorbed the desired protein, washing it carefully with the buffer, desorbing the absorbed protein by a solution of a polycationic compound in the case of T or by an albumin solution in the case of AT or of the complex T-AT, and isolating the protein, if desired, by known means, such as, especially, freeze drying. The invention is useful for studying the mechanism of blood coagulation.