SEPARATING MATERIAL FOR LEUKOCYTIC CELL OF NONHUMAN ANIMAL ORIGIN, SEPARATOR THEREFOR AND SEPARATION THEREOF

摘要

PURPOSE:To obtain a material capable of readily, simply, quickly separating the title cells in high yield and purity by immobilizing on the surface of a water-insoluble solid matter such a carbohydrate as to preferably adsorb the B lymphocyte fraction in the lymphocytes of nonhuman animal origin rather than the other lymphocyte fractions. CONSTITUTION:A separating material 2 for lenkocytic cells of nonhuman animal origin is prepared by immobilizing on the surface of a water-insoluble solid matter (e.g. oxirane group-contg. acrylic beads) such a carbohydrate (e.g. alginic acid) as to preferably adsorb the B lymphocyte fraction in the lymphocytes of nonhuman animal origin rather than the other lymphocyte fractions. This material 2 is then filled between filters 3 within the real diameter part 6 of a column 1 having said real diameter part 6 and a contracted diameter part 7, and a suspension of leukocytic cells of nonhuman animal origin is passed through said material 2 to selectively scavenge the granular spheres, single spheres and B lymphocytes present in this suspension to effect recovery of a T lymphocyte-rich non-scavenged liquid fraction via an outlet 5 into a test tube 8 through a stopcock 4 operation.