发明名称 |
METHOD FOR PRODUCING THERMALLY STABLE ALPHA-AMYLASE |
摘要 |
A process for the production of thermally stable alpha-amylase comprising the steps of: cultivating B. licheniformis ATCC 53,722 in a nutrient medium which contains as a primary carbon source a carbohydrate which normally represses alpha-amylase synthesis, selected from the group consisting of dextrose, starch hydrolysate, glycerol, xylose, mannose, sucrose and mixtures thereof; feeding said primary carbon source to said medium during cultivation; maintaining the level of carbon dioxide evolution from the medium during cultivation between about 4.5 % and 6.5 % of the off-gas when the air flow is 0.5 to 1.0 vvm by controlling the rate at which the primary carbon source is fed to said medium during cultivation; and maintaining the pH of the medium between 6.5 and 7.0. |
申请公布号 |
WO8912679(A1) |
申请公布日期 |
1989.12.28 |
申请号 |
WO1989US02683 |
申请日期 |
1989.06.19 |
申请人 |
GREAT LAKES CHEMICAL CORPORATION |
发明人 |
CHEN, JAMES, C.;LAMM, WILLIAM, R.;RACINE, FRANCIS, M.;TURNER, HAL, R. |
分类号 |
C12N9/28;C12R1/10;(IPC1-7):C12N9/28 |
主分类号 |
C12N9/28 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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