Method and system for the production of non-degraded proteins from mammalian cells.

摘要

A method for producing polypeptides said method comprising:perfusing culture media, through a microcarrier bead matrix supporting growth of mammalian cells capable of secreting the polypeptides into the culture media, wherein said mammalian cells are capable of effecting bead-to-bead transfer in the absence of proteolytic enzyme treatment, mechanical disruption, and calcium deprivation; collecting conditioned media from the reactor; and separating the polypeptides from the collected conditioned media.